6I5V

BlMnBP1 binding protein of an ABC transporter from Bifidobacterium animalis subsp. lactis ATCC27673 in complex with mannotriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9998
Spacegroup name	P 21 21 21
Unit cell lengths	56.330, 75.910, 102.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.345 - 2.010
R-factor	0.1961
Rwork	0.192
R-free	0.26490
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.345	2.082
High resolution limit [Å]	2.010	2.010
Rmerge	0.089	1.015
Rpim	0.042	0.475
Number of reflections	29709	2838
<I/σ(I)>	11.66	1.33
Completeness [%]	98.8	97.02
Redundancy	5.1	5.2
CC(1/2)	0.999	0.656

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	278	Mother liquor 0.1 M MES pH 5.5, 0.05 M Zn-acetate and 18% PEG 8000 Protein 15 mg /ml in 10 mM MES pH 6.5 and 150 mM NaCl Drops set up 1 to 1