6I5R

BlMnBP1 binding protein of an ABC transporter from Bifidobacterium animalis subsp. lactis ATCC27673 in complex with mannobiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.99985
Spacegroup name	P 21 21 21
Unit cell lengths	55.560, 74.500, 102.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.832 - 1.600
R-factor	0.2182
Rwork	0.217
R-free	0.24620
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.13_2998)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.832	1.657
High resolution limit [Å]	1.600	1.600
Rmerge	0.204	2.074
Rpim	0.214	0.653
Number of reflections	56799	5602
<I/σ(I)>	8.57
Completeness [%]	99.8	99.98
Redundancy	11.8	10.8
CC(1/2)	0.997	0.456

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	278	Mother liquor: 0.1 M MES pH 5.5, 0.05 M Zn-acetate and 18% PEG 8000 Protein conc 15 mg/ml in 10 mM MES pH 6.5 and 150 mM NaCl + 1mM Mannobiose Drops setup 1 to 1