6I5O
Crystal structure of SPBc2 prophage-derived protein YomS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.000, 52.160, 106.740 |
| Unit cell angles | 90.00, 95.97, 90.00 |
Refinement procedure
| Resolution | 53.140 - 1.330 |
| R-factor | 0.1554 |
| Rwork | 0.154 |
| R-free | 0.17770 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.898 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.6.2) |
| Phasing software | CRANK2 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 53.140 | 53.080 | 1.360 |
| High resolution limit [Å] | 1.330 | 5.950 | 1.330 |
| Rmerge | 0.088 | 0.033 | 1.397 |
| Rmeas | 0.098 | 0.037 | 1.564 |
| Rpim | 0.044 | 0.017 | 0.693 |
| Number of reflections | 134241 | 1574 | 9884 |
| <I/σ(I)> | 9.8 | ||
| Completeness [%] | 99.9 | 99.2 | 100 |
| Redundancy | 4.9 | 4.7 | 5 |
| CC(1/2) | 0.998 | 0.998 | 0.498 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M cacodylate pH 5.5, 0.2 M ammonium nitrate, 18 % (w/v) PEG Smear Low |
