6I5F
Crystal structure of DNA-free E.coli MutS P839E dimer mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2007-06-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.934 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 113.375, 113.525, 158.904 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.610 - 2.600 |
R-factor | 0.209 |
Rwork | 0.206 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wb9 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.270 |
Data reduction software | MOSFLM (6.2.6) |
Data scaling software | Aimless (0.3.11) |
Phasing software | PHASER (2.0) |
Refinement software | BUSTER |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 80.220 | 80.220 | 2.670 |
High resolution limit [Å] | 2.600 | 11.630 | 2.600 |
Rmerge | 0.299 | 0.073 | 1.194 |
Rmeas | 0.344 | 0.085 | 1.374 |
Rpim | 0.167 | 0.043 | 0.673 |
Total number of observations | 253695 | ||
Number of reflections | 62205 | 731 | 4381 |
<I/σ(I)> | 4.6 | ||
Completeness [%] | 98.1 | 94.3 | 94.9 |
Redundancy | 4.1 | 3.7 | 4 |
CC(1/2) | 0.957 | 0.990 | 0.110 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 0.1 M Hepes 7.0 7 % Dioxone 1.4 M Ammonium Sulfate |