6I3P

Crystal structure of DEAH-box ATPase Prp22 with bound ssRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9919
Spacegroup name	P 21 21 21
Unit cell lengths	139.790, 140.500, 159.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.240 - 2.750
R-factor	0.2233
Rwork	0.222
R-free	0.25310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fa5
RMSD bond length	0.015
RMSD bond angle	1.772
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.240	50.000	2.870
High resolution limit [Å]	2.750	49.240	2.750
Rmerge	0.110		1.469
Rmeas	0.118		1.567
Total number of observations	639883
Number of reflections	82130		9753
<I/σ(I)>	14.28		1.51
Completeness [%]	99.9		100
Redundancy	7.791		8.154
CC(1/2)	0.999		0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	100mM Bis-Tris propane pH8.5, 250mM NaF, 22% PEG3350