6I38
Crystal structure of nv2Pizza6-AYW, a circularly permuted designer protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-17 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.00003 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.295, 57.397, 69.827 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.340 - 1.580 |
R-factor | 0.1708 |
Rwork | 0.169 |
R-free | 0.20230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6f0q |
RMSD bond length | 0.004 |
RMSD bond angle | 0.766 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.340 | 1.610 |
High resolution limit [Å] | 1.580 | 1.580 |
Number of reflections | 27640 | |
<I/σ(I)> | 17.7 | |
Completeness [%] | 99.4 | |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292.15 | 21% (w/v) PEG3350 0.1M Bis-Tris pH5.5 +20% Glycerol |