6I2V
Pilotin from Vibrio vulnificus type 2 secretion system, EpsS.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-23 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 35.830, 83.210, 90.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.390 - 1.750 |
| R-factor | 0.18546 |
| Rwork | 0.179 |
| R-free | 0.22787 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ftf |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.397 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | Arcimboldo |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.390 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 14037 | |
| <I/σ(I)> | 14.14 | 2.12 |
| Completeness [%] | 99.3 | 95.8 |
| Redundancy | 16 | |
| CC(1/2) | 0.999 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293.15 | 0.1M tris Hcl pH 7.5 1.9M Amonium Sulfate 2.5% (v/v) PEG400 |
