6I1Z
Outward facing structure of apo CST
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.580, 69.460, 102.030 |
| Unit cell angles | 82.83, 83.43, 68.22 |
Refinement procedure
| Resolution | 21.950 - 3.400 |
| R-factor | 0.2687 |
| Rwork | 0.268 |
| R-free | 0.28590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6i1r |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.311 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.6.3) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.770 | 3.680 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.122 | 1.323 |
| Rmeas | 0.133 | 1.458 |
| Rpim | 0.052 | 0.590 |
| Number of reflections | 17696 | 3623 |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 99.2 | 97.7 |
| Redundancy | 6.4 | 5.1 |
| CC(1/2) | 0.984 | 0.532 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 35 % (v/v) PEG 400, 0.1 M NaCl, 0.1 M MES |
