6I1C
Crystal structure of Chlamydomonas reinhardtii thioredoxin f2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-17 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9762 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 65.383, 97.475, 139.545 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.850 - 2.010 |
R-factor | 0.2282 |
Rwork | 0.226 |
R-free | 0.26280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Homology modelling by PHYRE2 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.968 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.850 | 2.082 |
High resolution limit [Å] | 2.010 | 2.010 |
Number of reflections | 29702 | |
<I/σ(I)> | 6.69 | |
Completeness [%] | 98.7 | |
Redundancy | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM HEPES-NaOH, pH 7.5, 2% PEG 400, 2.0 M ammonium sulfate |