6I1A
Crystal structure of rutinosidase from Aspergillus niger
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.068, 97.359, 139.259 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.600 - 1.270 |
| R-factor | 0.15949 |
| Rwork | 0.159 |
| R-free | 0.17822 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.925 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Rmeas | 0.068 | 1.446 |
| Number of reflections | 180049 | 22781 |
| <I/σ(I)> | 9.58 | 0.78 |
| Completeness [%] | 94.6 | 74.8 |
| Redundancy | 2.98 | 2.19 |
| CC(1/2) | 0.999 | 0.359 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.5 | 291 | 20 mM Amonium Sulfate, 0.1 M Bis-Tris, 25% PEG 3350 |
