6I0V
Crystal structure of DmTailor in complex with CACAGU RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-24 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00004 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 60.077, 60.077, 162.293 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.799 - 1.851 |
| R-factor | 0.1962 |
| Rwork | 0.195 |
| R-free | 0.22580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6i0s |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.948 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.800 | 1.960 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 29819 | |
| <I/σ(I)> | 24.17 | |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 19.3 | 18.9 |
| CC(1/2) | 1.000 | 0.421 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 35% (v/v) glycerol ethoxylate, 75 mM sodium malonate, pH7.0 |
