6I09
Crystal structure of RlpA SPOR domain from Pseudomonas aeruginosa in complex with denuded glycan obtained by soaking
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-24 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979257 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 67.433, 68.151, 38.994 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.930 - 1.480 |
| R-factor | 0.1704 |
| Rwork | 0.169 |
| R-free | 0.19880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6i05 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.858 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.934 | 1.530 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.054 | 0.854 |
| Rpim | 0.020 | 0.319 |
| Number of reflections | 15044 | 1475 |
| <I/σ(I)> | 14.8 | 1.8 |
| Completeness [%] | 97.8 | |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.15 M NaF and 16% (w/v) PEG3350 |
