6I03
D10N variant of beta-phosphoglucomutase from Lactococcus lactis complexed with tetrafluoroaluminate and beta-G6P to 1.02 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.520, 54.280, 104.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.330 - 1.020 |
| R-factor | 0.15265 |
| Rwork | 0.151 |
| R-free | 0.17470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wf6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.507 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.520 | 1.050 |
| High resolution limit [Å] | 1.020 | 1.020 |
| Rmerge | 0.045 | 0.917 |
| Rmeas | 0.054 | 1.209 |
| Rpim | 0.021 | 0.589 |
| Number of reflections | 106736 | 6606 |
| <I/σ(I)> | 16.1 | 1.3 |
| Completeness [%] | 97.6 | 82.5 |
| Redundancy | 6.5 | 3.7 |
| CC(1/2) | 0.999 | 0.542 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 291 | 24-34% PEG 4000 200 mM sodium acetate 50 mM TRIS, pH 7.5 |
