6HZL
Crystal structure of redox-inhibited phosphoribulokinase from Synechococcus sp. (strain PCC 6301), osmate derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.13937 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 141.432, 141.432, 206.563 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.770 |
| R-factor | 0.2363 |
| Rwork | 0.235 |
| R-free | 0.25890 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.897 |
| Data reduction software | XDS (VERSION Nov 1, 2016) |
| Data scaling software | Aimless (0.5.28) |
| Phasing software | HKL2Map |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.330 | 49.330 | 2.920 |
| High resolution limit [Å] | 2.770 | 8.760 | 2.770 |
| Rmerge | 0.226 | 0.094 | 2.465 |
| Rmeas | 0.228 | 0.094 | 2.517 |
| Rpim | 0.030 | 0.013 | 0.502 |
| Total number of observations | 1062487 | 38830 | 68326 |
| Number of reflections | 20468 | 706 | 2938 |
| <I/σ(I)> | 17.4 | 53.9 | 1.7 |
| Completeness [%] | 100.0 | 99.5 | 100 |
| Redundancy | 51.9 | 55 | 23.3 |
| CC(1/2) | 0.999 | 0.999 | 0.706 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 277 | 20 % PEG-3350, 200 mM ammonium formate |
