6HYX
THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT Y197F-P250C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2015-10-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9785 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.810, 134.230, 160.300 |
| Unit cell angles | 90.00, 102.09, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.370 | 3.060 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 75432 | |
| <I/σ(I)> | 12.9 | 3.5 |
| Completeness [%] | 99.5 | 99 |
| Redundancy | 2.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4 | 293 | PH 4, VAPOR DIFFUSION, TEMPERATURE 293K.12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4 |
