6HYO
Structure of ULK1 LIR motif bound to GABARAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9159 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 100.720, 100.720, 100.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.119 - 1.070 |
| R-factor | 0.1425 |
| Rwork | 0.142 |
| R-free | 0.16080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gnu |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.392 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.120 | 1.100 |
| High resolution limit [Å] | 1.070 | 1.070 |
| Rmerge | 0.040 | 1.650 |
| Rmeas | 0.050 | 1.710 |
| Rpim | 0.010 | 0.440 |
| Number of reflections | 74520 | 7428 |
| <I/σ(I)> | 23.9 | 1.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 18.6 | 14.6 |
| CC(1/2) | 1.000 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M MgCl2. 0.1 M Na cacodylate pH 6.5, 20% PEG1000 |
