6HYL

Structure of PCM1 LIR motif bound to GABARAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-18
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	53.696, 65.977, 95.722
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.320 - 1.559
R-factor	0.2013
Rwork	0.199
R-free	0.24690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gnu
RMSD bond length	0.002
RMSD bond angle	0.514
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.320	1.610
High resolution limit [Å]	1.550	1.550
Rmerge	0.050	0.970
Rmeas	0.060	1.080
Rpim	0.020	0.470
Number of reflections	49150	4840
<I/σ(I)>	11.1	1.2
Completeness [%]	99.0	95.7
Redundancy	5.2	5.1
CC(1/2)	0.998	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M CaCl2, 0.1 M HEPES pH 7, 20% w/v PEG 6000