6HYJ

PSPH Human phosphoserine phosphatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97857
Spacegroup name	C 2 2 21
Unit cell lengths	48.670, 128.970, 155.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.230 - 1.929
R-factor	0.203
Rwork	0.200
R-free	0.26340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nnl
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.230	1.998
High resolution limit [Å]	1.929	1.929
Rmerge	0.030	0.553
Rmeas	0.042	0.782
Rpim	0.030	0.553
Number of reflections	36957	3228
<I/σ(I)>	11.27	0.96
Completeness [%]	98.7	87.57
Redundancy	2	1.9
CC(1/2)	0.999	0.589

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	CaCl2 0.15 M; sodium cacodylate 0.1 M pH 6.5; PEG 2000 20%