6HXY
Crystal structure of the head and coiled-coil domains of zebrafish CCDC61
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 93.088, 100.560, 135.759 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.879 - 2.900 |
| R-factor | 0.2057 |
| Rwork | 0.200 |
| R-free | 0.25810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hxv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.203 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.310 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.113 | 0.849 |
| Rmeas | 0.124 | 0.930 |
| Rpim | 0.051 | 0.375 |
| Number of reflections | 14498 | 2302 |
| <I/σ(I)> | 9.4 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.9 | 6.1 |
| CC(1/2) | 0.999 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 1 M LiCl, 100 mM Citric acid |
