6HXV

Crystal structure of the head and coiled-coil domains of zebrafish CCDC61 (F129E/D130A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-11-01
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	55.360, 76.310, 83.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.155 - 1.970
R-factor	0.1763
Rwork	0.173
R-free	0.23790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6hxt
RMSD bond length	0.008
RMSD bond angle	1.098
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.810	2.020
High resolution limit [Å]	1.970	1.970
Rmerge	0.102	0.868
Rmeas	0.110	0.937
Rpim	0.041	0.350
Number of reflections	25068	1702
<I/σ(I)>	12.6	2.4
Completeness [%]	98.2	96.6
Redundancy	7	6.9
CC(1/2)	0.997	0.756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	8%(w/v) PEG6,000, 100 mM Bicine, 3% Triemethylamine N-Oxide