6HXV
Crystal structure of the head and coiled-coil domains of zebrafish CCDC61 (F129E/D130A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-11-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.360, 76.310, 83.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.155 - 1.970 |
| R-factor | 0.1763 |
| Rwork | 0.173 |
| R-free | 0.23790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hxt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.098 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.810 | 2.020 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.102 | 0.868 |
| Rmeas | 0.110 | 0.937 |
| Rpim | 0.041 | 0.350 |
| Number of reflections | 25068 | 1702 |
| <I/σ(I)> | 12.6 | 2.4 |
| Completeness [%] | 98.2 | 96.6 |
| Redundancy | 7 | 6.9 |
| CC(1/2) | 0.997 | 0.756 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 8%(w/v) PEG6,000, 100 mM Bicine, 3% Triemethylamine N-Oxide |
