6HXT
Crystal structure of the head domain of human CCDC61
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 36.877, 68.222, 180.728 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.680 - 2.550 |
| R-factor | 0.2131 |
| Rwork | 0.208 |
| R-free | 0.25600 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.680 | 2.660 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.077 | 0.568 |
| Rmeas | 0.086 | 0.630 |
| Rpim | 0.037 | 0.266 |
| Number of reflections | 15498 | 1872 |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 99.1 | 99.7 |
| Redundancy | 5 | 5.2 |
| CC(1/2) | 0.998 | 0.932 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | 8%(w/v) PEG6,000, 100 mM Tris-HC |
