6HX0

Cell wall binding domain of endolysin from Listeria phage A500.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-19
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 32
Unit cell lengths	49.579, 49.579, 63.766
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.940 - 1.590
R-factor	0.1746
Rwork	0.148
R-free	0.20350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xov
RMSD bond length	0.001
RMSD bond angle	0.392
Data reduction software	MOSFLM (v7)
Data scaling software	SCALA (1.10.28)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.700	1.670
High resolution limit [Å]	1.587	1.587
Rmerge	0.159	1.005
Rmeas	0.168	1.073
Rpim	0.055	0.366
Number of reflections	23591	3411
<I/σ(I)>	8.5	2.4
Completeness [%]	99.1	99.4
Redundancy	8.99	8.28
CC(1/2)	0.993	0.349

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	292	100 mM HEPES buffer, pH 7.5, 3.3 M Sodium Chloride, 1% (v/v) glycerol. Mixed 1:1 with protein solution (6.6-10 mg/ml; 20mM Tris, 150mM NaCl, pH 7.4)