6HWP

Structure of A3_bGFPD, an artificial bi-domain protein based on two different alphaRep domains : A3 and a GFP binding domain (bGFPD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.970
Spacegroup name	C 2 2 21
Unit cell lengths	66.910, 89.000, 143.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.500 - 2.547
R-factor	0.2372
Rwork	0.236
R-free	0.26240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ltj
RMSD bond length	0.002
RMSD bond angle	0.493
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.500	50.000	2.700
High resolution limit [Å]	2.547	7.540	2.550
Rmerge	0.155	0.048	1.739
Rmeas	0.169	0.053	1.883
Number of reflections	14334	616	2230
<I/σ(I)>	9.24	31.27	1.09
Completeness [%]	98.9	99.5	98.6
Redundancy	6.61	6.07	6.75
CC(1/2)	0.997	0.997	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	1.6M Tri-sodium citrate