6HWN
Structure of Thermus thermophilus ClpP in complex with a tripeptide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-09 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9660 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.976, 162.786, 107.948 |
| Unit cell angles | 90.00, 116.34, 90.00 |
Refinement procedure
| Resolution | 46.587 - 1.950 |
| R-factor | 0.1883 |
| Rwork | 0.186 |
| R-free | 0.21090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kti |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.956 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.590 | 1.972 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmeas | 0.078 | 1.093 |
| Number of reflections | 118066 | 11696 |
| <I/σ(I)> | 13.06 | 1.56 |
| Completeness [%] | 99.3 | 98.58 |
| Redundancy | 5.3 | 5.3 |
| CC(1/2) | 0.999 | 0.646 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | 100 mM Sodium acetate pH 4.8 and 30% PEG 400. |
