6HW2

The Crystal Structure of CaV beta4c in complex with HP1gamma chromo shadow domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-17
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	C 1 2 1
Unit cell lengths	115.026, 58.036, 57.546
Unit cell angles	90.00, 109.70, 90.00

Refinement procedure

Resolution	33.120 - 1.941
R-factor	0.1792
Rwork	0.177
R-free	0.20950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vyv
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.120	2.010
High resolution limit [Å]	1.941	1.941
Rmerge	0.047	0.404
Number of reflections	26484	2518
<I/σ(I)>	12.8
Completeness [%]	99.8
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	292	0.15 M NaCl, 12-14% PEG 3350 , pH 5.0, calcium acetate, VAPOR DIFFUSION, temperature 292K