6HW1
ROOM TEMPERATURE STRUCTURE OF LIPASE FROM T. LANUGINOSA AT 2.5 A RESOLUTION IN CHIPX MICROFLUIDIC DEVICE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 295 |
Detector technology | PIXEL |
Collection date | 2017-03-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 61 |
Unit cell lengths | 142.652, 142.652, 80.721 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.055 - 2.500 |
R-factor | 0.1733 |
Rwork | 0.172 |
R-free | 0.19940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gwl |
RMSD bond length | 0.003 |
RMSD bond angle | 0.518 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.060 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.086 | 0.900 |
Rmeas | 0.114 | 1.200 |
Rpim | 0.075 | 0.800 |
Number of reflections | 31982 | 3668 |
<I/σ(I)> | 6.3 | 0.8 |
Completeness [%] | 98.5 | 98.9 |
Redundancy | 3.2 | 3.1 |
CC(1/2) | 0.994 | 0.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | COUNTER-DIFFUSION | 7.5 | 293 | 300 mM Na/K Phosphate, 50 mM NaAc pH 4.5. COUNTER-DIFFUSION IN CHIPX MICROFLUIDIC DEVICE |