6HVO

Crystal structure of human PCNA in complex with three peptides of p12 subunit of human polymerase delta

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97925
Spacegroup name	P 21 21 21
Unit cell lengths	71.965, 83.888, 154.982
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.880 - 2.100
R-factor	0.1975
Rwork	0.195
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.445
Data reduction software	XDS
Data scaling software	Aimless (0.5.17)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	154.980	154.980	2.160
High resolution limit [Å]	2.100	8.910	2.100
Rmerge	0.166	0.096
Rmeas	0.173	0.101
Rpim	0.048	0.031	0.775
Total number of observations	718873
Number of reflections	55602	846	4493
<I/σ(I)>	10.7
Completeness [%]	100.0	99.5	100
Redundancy	12.9	10.1	13.2
CC(1/2)	0.996	0.991	0.447

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	Bis Tris 6.5 , Li2SO4 (0.2M), PEG 3350 24%