6HVO
Crystal structure of human PCNA in complex with three peptides of p12 subunit of human polymerase delta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.965, 83.888, 154.982 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.880 - 2.100 |
| R-factor | 0.1975 |
| Rwork | 0.195 |
| R-free | 0.24440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.445 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 154.980 | 154.980 | 2.160 |
| High resolution limit [Å] | 2.100 | 8.910 | 2.100 |
| Rmerge | 0.166 | 0.096 | |
| Rmeas | 0.173 | 0.101 | |
| Rpim | 0.048 | 0.031 | 0.775 |
| Total number of observations | 718873 | ||
| Number of reflections | 55602 | 846 | 4493 |
| <I/σ(I)> | 10.7 | ||
| Completeness [%] | 100.0 | 99.5 | 100 |
| Redundancy | 12.9 | 10.1 | 13.2 |
| CC(1/2) | 0.996 | 0.991 | 0.447 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | Bis Tris 6.5 , Li2SO4 (0.2M), PEG 3350 24% |
