6HVG
Crystal Structure of Truncated Alternansucrase from Leuconostoc mesenteroides NRRL B-1355
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-27 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 101.235, 134.804, 237.035 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.820 - 2.800 |
| R-factor | 0.2082 |
| Rwork | 0.207 |
| R-free | 0.23732 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lfc |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.265 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.820 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.088 | 0.571 |
| Number of reflections | 80120 | 11606 |
| <I/σ(I)> | 7.3 | 1.4 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 6.9 | |
| CC(1/2) | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 285 | PEG3350 14% 0.4M NaNO3 |
