6HVG

Crystal Structure of Truncated Alternansucrase from Leuconostoc mesenteroides NRRL B-1355

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-27
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 21 21 21
Unit cell lengths	101.235, 134.804, 237.035
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.820 - 2.800
R-factor	0.2082
Rwork	0.207
R-free	0.23732
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lfc
RMSD bond length	0.009
RMSD bond angle	1.265
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.820	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.088	0.571
Number of reflections	80120	11606
<I/σ(I)>	7.3	1.4
Completeness [%]	99.4	99.9
Redundancy	6.9
CC(1/2)		0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	285	PEG3350 14% 0.4M NaNO3