6HUN
Dimeric Archeal Rubisco from Hyperthermus butylicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 75.412, 69.866, 94.494 |
| Unit cell angles | 90.00, 112.62, 90.00 |
Refinement procedure
| Resolution | 43.613 - 1.802 |
| R-factor | 0.1807 |
| Rwork | 0.179 |
| R-free | 0.21630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1geh |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.310 | 49.310 | 1.840 |
| High resolution limit [Å] | 1.800 | 9.010 | 1.800 |
| Rmerge | 0.064 | 0.054 | 0.223 |
| Rmeas | 0.076 | 0.066 | 0.272 |
| Rpim | 0.041 | 0.037 | 0.153 |
| Number of reflections | 41694 | 356 | 2346 |
| <I/σ(I)> | 8.9 | ||
| Completeness [%] | 99.4 | 97.7 | 94.1 |
| Redundancy | 3.4 | 2.8 | 3 |
| CC(1/2) | 0.995 | 0.989 | 0.934 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M CaCl2, 20% (w/v) PEG6000, and 100 mM sodium acetate/acetic acid |
