6HUN

Dimeric Archeal Rubisco from Hyperthermus butylicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	I 1 2 1
Unit cell lengths	75.412, 69.866, 94.494
Unit cell angles	90.00, 112.62, 90.00

Refinement procedure

Resolution	43.613 - 1.802
R-factor	0.1807
Rwork	0.179
R-free	0.21630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1geh
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.310	49.310	1.840
High resolution limit [Å]	1.800	9.010	1.800
Rmerge	0.064	0.054	0.223
Rmeas	0.076	0.066	0.272
Rpim	0.041	0.037	0.153
Number of reflections	41694	356	2346
<I/σ(I)>	8.9
Completeness [%]	99.4	97.7	94.1
Redundancy	3.4	2.8	3
CC(1/2)	0.995	0.989	0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M CaCl2, 20% (w/v) PEG6000, and 100 mM sodium acetate/acetic acid