6HU6
Structure of ARF1 RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-10-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.000 |
Spacegroup name | H 3 2 |
Unit cell lengths | 43.803, 43.803, 452.087 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.802 - 1.904 |
R-factor | 0.2274 |
Rwork | 0.226 |
R-free | 0.24880 |
RMSD bond length | 0.019 |
RMSD bond angle | 2.407 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.000 | 2.020 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 25323 | |
<I/σ(I)> | 13.52 | |
Completeness [%] | 99.9 | |
Redundancy | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 1.7 M (NH4)2SO4, 100 mM Tri-sodium citrate pH 6.2 |