Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2005-07-23 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.98400 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.890, 61.710, 110.360 |
| Unit cell angles | 90.00, 99.33, 90.00 |
Refinement procedure
| Resolution | 19.950 - 1.961 |
| R-factor | 0.1655 |
| Rwork | 0.163 |
| R-free | 0.21350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v8z |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.950 | 2.031 |
| High resolution limit [Å] | 1.960 | 1.961 |
| Rmerge | 0.061 | 0.427 |
| Rmeas | 0.071 | 0.500 |
| Rpim | 0.037 | 0.258 |
| Number of reflections | 53095 | |
| <I/σ(I)> | 15.05 | |
| Completeness [%] | 99.4 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 30% PEG 4000, 100 mM Tris pH 8.5 and 100 mM NaCl |
