6HS6
C-terminal domain of the TssA component of the type VI secretion system from Burkholderia cenocepacia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-31 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.70001 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 46.330, 201.700, 263.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.490 - 3.080 |
| R-factor | 0.20048 |
| Rwork | 0.198 |
| R-free | 0.24245 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.621 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.490 | 3.160 |
| High resolution limit [Å] | 3.080 | 3.080 |
| Rmerge | 0.131 | 0.786 |
| Rpim | 0.040 | 0.251 |
| Number of reflections | 23602 | 1709 |
| <I/σ(I)> | 17.5 | 3.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.6 | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.1M sodium chloride, 0.1M Tris pH8.0, 15% (v/v) ethanol, 5% (v/v) MPD |
