6HRK
Structure of a far-red fluorescent biliprotein derived from a far-red induced allophycocyanin F subunit from a thermophilic cyanobacterium Chroococcidiopsis thermalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-05 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.248, 74.823, 61.031 |
| Unit cell angles | 90.00, 90.67, 90.00 |
Refinement procedure
| Resolution | 47.390 - 2.520 |
| R-factor | 0.27695 |
| Rwork | 0.274 |
| R-free | 0.33173 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cpc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.479 |
| Data reduction software | xia2 |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.610 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.296 | 0.886 |
| Number of reflections | 17506 | 1603 |
| <I/σ(I)> | 4.28 | 1.24 |
| Completeness [%] | 93.0 | 90 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% (v/v) 2-propanol 0.1 M MES monohydrate pH 6.0 20% (w/v) PEGMME 2000 |
