6HQZ
Crystal structure of the type III effector protein AvrRpt2 from Erwinia amylovora, a C70 family cysteine protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-21 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.064 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.313, 63.884, 85.983 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.320 - 1.800 |
| R-factor | 0.2106 |
| Rwork | 0.209 |
| R-free | 0.23315 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.865 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.320 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmeas | 0.054 | 0.664 |
| Number of reflections | 28980 | 2004 |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 99.0 | 94.76 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 9 | 293 | 100 mM TRIS-HCL pH 9.0 10% glycerol, 100 mM sodium cacodylate, 15% PEG 6K |
