6HPG
Arabidopsis OM64 TPR domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2015-11-24 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.966000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.388, 61.135, 106.563 |
| Unit cell angles | 87.57, 87.99, 78.26 |
Refinement procedure
| Resolution | 29.910 - 2.000 |
| R-factor | 0.1864 |
| Rwork | 0.182 |
| R-free | 0.26530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vyi |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.484 |
| Data reduction software | ADDREF |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | ACORN |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.910 | 29.070 | 2.110 |
| High resolution limit [Å] | 2.000 | 6.320 | 2.000 |
| Rmerge | 0.068 | 0.027 | 0.454 |
| Rmeas | 0.097 | 0.038 | 0.642 |
| Rpim | 0.068 | 0.027 | 0.454 |
| Number of reflections | 45460 | 1392 | 6451 |
| <I/σ(I)> | 5.8 | ||
| Completeness [%] | 92.7 | 89.6 | 89.3 |
| Redundancy | 1.7 | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.1 M Citric acid, pH 3.5, 25 % (w/v) PEG 3350 |
