6HON

Drosophila NOT4 CBM peptide bound to human CAF40

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	I 21 21 21
Unit cell lengths	85.630, 90.320, 196.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.250 - 2.200
R-factor	0.1947
Rwork	0.193
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fv2 chain A
RMSD bond length	0.010
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.250
High resolution limit [Å]	2.200	2.200
Number of reflections	39129	2831
<I/σ(I)>	16.6	1.6
Completeness [%]	99.9
Redundancy	8.9
CC(1/2)	1.000	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.9 M K2HPO4, 0.3 M NaH2PO4