6HOA
TRANSCRIPTIONAL REPRESSOR ETHR FROM MYCOBACTERIUM TUBERCULOSIS IN COMPLEX WITH BDM44830
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 121.930, 121.930, 33.751 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.110 - 1.500 |
| R-factor | 0.18932 |
| Rwork | 0.188 |
| R-free | 0.21443 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u9n |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.827 |
| Data reduction software | iMOSFLM |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.110 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.105 | 1.866 |
| Number of reflections | 41386 | 5869 |
| <I/σ(I)> | 18.4 | 1.5 |
| Completeness [%] | 99.7 | 98.6 |
| Redundancy | 11.9 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.7 | 293 | 1.4-1.6 ammonium sulfate, 15% glycerol, 100 mM MES |
