6HNN
Crystal structure of wild-type IdmH, a putative polyketide cyclase from Streptomyces antibioticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.684, 103.519, 99.584 |
| Unit cell angles | 90.00, 91.63, 90.00 |
Refinement procedure
| Resolution | 51.760 - 2.700 |
| R-factor | 0.21232 |
| Rwork | 0.211 |
| R-free | 0.24421 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hnm |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.472 |
| Data reduction software | TRUNCATE |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.760 | 2.820 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.086 | |
| Rmeas | 0.097 | |
| Rpim | 0.046 | |
| Number of reflections | 37144 | 4498 |
| <I/σ(I)> | 11.4 | 1.4 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 4.5 | 4.3 |
| CC(1/2) | 0.998 | 0.655 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.2 M calcium acetate, 0.1 MES, 18.6% (w/v) polyethylene glycol 8000 |
