6HNM

Crystal structure of IdmH 96-104 loop truncation variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976
Spacegroup name	F 2 3
Unit cell lengths	152.686, 152.686, 152.686
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.340 - 2.000
R-factor	0.22318
Rwork	0.221
R-free	0.26754
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6hnl
RMSD bond length	0.008
RMSD bond angle	1.261
Data reduction software	TRUNCATE
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.340	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.046
Rmeas	0.053
Rpim	0.026
Number of reflections	19992	1475
<I/σ(I)>	12.7	1.4
Completeness [%]	99.9	99.9
Redundancy	4.1	4.1
CC(1/2)	0.999	0.516

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	3.6-4 M sodium formate, 10-15% (v/v) glycerol