6HNM
Crystal structure of IdmH 96-104 loop truncation variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 152.686, 152.686, 152.686 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.340 - 2.000 |
| R-factor | 0.22318 |
| Rwork | 0.221 |
| R-free | 0.26754 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hnl |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.261 |
| Data reduction software | TRUNCATE |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.340 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.046 | |
| Rmeas | 0.053 | |
| Rpim | 0.026 | |
| Number of reflections | 19992 | 1475 |
| <I/σ(I)> | 12.7 | 1.4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.1 | 4.1 |
| CC(1/2) | 0.999 | 0.516 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 3.6-4 M sodium formate, 10-15% (v/v) glycerol |
