6HNL
Selenomethionine derivative of IdmH 96-104 loop truncation variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-30 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 152.590, 152.590, 152.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.300 - 2.200 |
| R-factor | 0.22882 |
| Rwork | 0.227 |
| R-free | 0.27284 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.371 |
| Data reduction software | TRUNCATE |
| Data scaling software | Aimless |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.300 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.118 | |
| Rmeas | 0.120 | |
| Rpim | 0.022 | |
| Number of reflections | 15068 | 1285 |
| <I/σ(I)> | 20.8 | 1.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 30.6 | 30.6 |
| CC(1/2) | 0.999 | 0.812 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 3.6-4 M sodium formate, 10-15% (v/v) glycerol |
