6HNB
Crystal structure of aminotransferase Aro8 from Candida albicans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.3 |
| Synchrotron site | BESSY |
| Beamline | 14.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.895 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.371, 102.258, 147.051 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.290 - 1.960 |
| R-factor | 0.17847 |
| Rwork | 0.177 |
| R-free | 0.23294 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4je5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.528 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.080 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.148 | |
| Number of reflections | 76434 | 11840 |
| <I/σ(I)> | 13.05 | 2.03 |
| Completeness [%] | 99.4 | 96.6 |
| Redundancy | 7.1 | 7 |
| CC(1/2) | 0.997 | 0.697 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.2 M MgCl2, 0.1 M Bis-Tris pH 5.5, 25% PEG 3350 |
