6HMJ

Structure of an RNA-binding Light-Oxygen-Voltage Receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91814
Spacegroup name	P 21 21 21
Unit cell lengths	67.013, 150.345, 219.783
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.127 - 2.510
R-factor	0.2437
Rwork	0.243
R-free	0.25410
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.634
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	SHELXCD
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.127	2.600
High resolution limit [Å]	2.510	2.510
Rmeas	0.228	2.321
Rpim	0.108	1.074
Number of reflections	75981
<I/σ(I)>	8
Completeness [%]	98.7
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.3	0.1 M bicine, pH 9.2, 15% (w/v) PEG 20,000, 2% dioxane, 0.8 M imidazole acetate