6HM3

Crystal structure of Rad4 BRCT1,2 in complex with a Sld3 phosphopeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	39.400, 59.170, 120.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.204 - 1.773
R-factor	0.193450598832
Rwork	0.192
R-free	0.22784
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bmc
RMSD bond length	0.007
RMSD bond angle	0.904
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.204	1.820
High resolution limit [Å]	1.770	1.770
Number of reflections	27589
<I/σ(I)>	15.7
Completeness [%]	98.3
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		287.15	Unknown