6HLV

Crystal structure of human ACBD3 GOLD domain in complex with 3A protein of poliovirus-1 (L24A mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	C 1 2 1
Unit cell lengths	90.656, 53.787, 62.813
Unit cell angles	90.00, 107.64, 90.00

Refinement procedure

Resolution	43.200 - 2.500
R-factor	0.2163
Rwork	0.215
R-free	0.24140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lz1
RMSD bond length	0.003
RMSD bond angle	0.824
Data reduction software	XDS (11-Sep-2018)
Data scaling software	XDS (11-Sep-2018)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.200	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.117	1.022
Rmeas	0.140
Number of reflections	10009	983
<I/σ(I)>	7.43	1.24
Completeness [%]	98.9	98.89
Redundancy	3.4	3.5
CC(1/2)	0.993	0.542

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	12,5% w/v PEG 4000, 20% v/v 1,2,6-hexanetriol, 4% v/v tert-butanol, 1 mM rubidium chloride, 1 mM strontium chloride, 1 mM cesium acetate, 1 mM barium acetate, 100 mM GlyGly/AMPD pH 8.5