6HLT

Crystal structure of human ACBD3 GOLD domain in complex with 3A protein of rhinovirus-14 (HRV14)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 1 21 1
Unit cell lengths	54.429, 78.955, 70.587
Unit cell angles	90.00, 112.38, 90.00

Refinement procedure

Resolution	42.440 - 2.815
R-factor	0.2118
Rwork	0.210
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lz1
RMSD bond length	0.008
RMSD bond angle	1.022
Data reduction software	XDS (11-Sep-2018)
Data scaling software	XDS (11-Sep-2018)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.440	2.916
High resolution limit [Å]	2.815	2.815
Number of reflections	13308	1286
<I/σ(I)>	9.85	1.61
Completeness [%]	98.4	95.33
Redundancy	3.8	3.7
CC(1/2)	0.995	0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	10% w/v PEG 8000, 20% v/v ethylene glycol, 30 mM MgCl2, 30 mM CaCl2, 100 mM bicine/Tris pH 8.5