6HLN

Crystal structure of human ACBD3 GOLD domain in complex with 3A protein of enterovirus-D68

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	C 1 2 1
Unit cell lengths	96.879, 55.889, 64.493
Unit cell angles	90.00, 112.05, 90.00

Refinement procedure

Resolution	47.450 - 2.100
R-factor	0.2222
Rwork	0.221
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lz1
RMSD bond length	0.007
RMSD bond angle	1.065
Data reduction software	XDS (11-Sep-2018)
Data scaling software	XDS (11-Sep-2018)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.450	2.175
High resolution limit [Å]	2.100	2.100
Number of reflections	18521	1848
<I/σ(I)>	10.78	1.21
Completeness [%]	98.3	99.09
Redundancy	3.8	3.8
CC(1/2)	0.997	0.556

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	10% w/v PEG 8000, 6% v/v 1,5-pentanediol, 14% v/v PEG 200, 10 mM spermine, 10 mM spermidine, 10 mM DL-ornithine, 10 mM 1,4-diaminobutane, 300 mM NaCl, 100 mM GlyGly/AMPD pH 8.5