6HL9
Crystal Structure of the CsiD Glutarate Hydroxylase in complex with Succinate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 123.034, 123.034, 138.327 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.109 - 2.300 |
| R-factor | 0.1869 |
| Rwork | 0.186 |
| R-free | 0.21030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gpe |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.109 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 47789 | |
| <I/σ(I)> | 22.43 | |
| Completeness [%] | 98.3 | |
| Redundancy | 26.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 1.0 M Succinic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0 |
