6HKG
Structure of FISW84 Fab Fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 130.160, 130.160, 167.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.749 - 1.870 |
| R-factor | 0.1835 |
| Rwork | 0.183 |
| R-free | 0.19950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vxs 2vxv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.902 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.750 | 1.920 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmeas | 0.086 | |
| Number of reflections | 118143 | 8595 |
| <I/σ(I)> | 11.5 | 1 |
| Completeness [%] | 99.6 | |
| Redundancy | 5.9 | 5.7 |
| CC(1/2) | 0.998 | 0.288 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 298 | 2.04 M ammonium sulfate, 0.1 M sodium acetate pH 4.6 Cryoprotected in 2.1 M ammonium sulfate, 0.1 M sodium acetate pH 4.6 and 25 % (v/v) ethylene glycol |
