6HJT

The X-ray structure of the horse spleen ferritin nanocage containing Pt, obtained upon encapsulation of a Pt(II) terpyridine compound within the protein cage

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.06
Spacegroup name	F 4 3 2
Unit cell lengths	181.156, 181.156, 181.156
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.300 - 1.330
R-factor	0.129
Rwork	0.127
R-free	0.15700
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	BUSTER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.000	1.364
High resolution limit [Å]	1.327	1.327
Rmerge	0.144
Rmeas	0.148
Rpim	0.034	0.868
Number of reflections	57025	2860
<I/σ(I)>	13.5
Completeness [%]	96.5	62.3
Redundancy	18.5	19.2
CC(1/2)	0.998	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	0.6 M (NH4)2SO4, 0.1 M Tris HCl pH 7.4, 60 mM CdSO4