6HJM
Myxococcus xanthus MglB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978570 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 119.783, 139.599, 179.416 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.410 - 2.390 |
| R-factor | 0.25 |
| Rwork | 0.249 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t1s |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.410 | 2.480 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.087 | 1.925 |
| Rmeas | 0.092 | 2.043 |
| Number of reflections | 118348 | 1124 |
| <I/σ(I)> | 15.36 | 1.1 |
| Completeness [%] | 73.0 | 97 |
| Redundancy | 9 | 8.9 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M MES, 0.15M Ammonium sulphate, 15% PEG 4000 |
